The Arsenal defender was probably the one player the team didn’t want to miss going into the country’s biggest game in 23 years.
Tierney has emerged as a hugely influential player as part of the back three. He is more than a defender, he is a leader and a key attacking threat, combining excellently down the left with captain Andy Robertson.
According to STV Sport’s Jamie Borthwick, the 24-year-old has been doing a reduced workload in training.
Scotland head coach Steve Clarke confirmed he has been left out with Friday’s England game in mind.
"Kieran picked up a little niggle during the week in training - not ready for this one, hopefully ready for next one," he said.
Clarke has lined up with Liam Cooper, Grant Hanley and Jack Hendry in the centre of defence. Che Adams is also not starting with Ryan Christie, Stuart Armstrong and John McGinn supporting Lyndon Dykes.
Scotland kick-off against Czech Republic at 2pm.