The world-famous zoo has more than 100 gentoos, kings and rockhoppers in its enclosures - all of which can be viewed on a live streaming webcam.
But Twitter user @Jazzmellodi worried the penguin population might have fallen by one when she tweeted a screenshot of the webcam that appeared to show a bird lying on its side.
Jazz asked the zoo: “Is this penguin okay? He hasn't moved.”
After a quick penguin headcount by its keepers, Edinburgh Zoo retweeted the query, writing: “Hi Jazz, we do have rockhoppers but that's just a rock…”
“Well this is embarrassing,” Jazz replied, adding: “back off to specsavers I go.”
You can keep up to date with what the penguins at Edinburgh Zoo get up to - and make sure they’re all upright and conscious - by watching the live stream from their webcam here.