Lisa Donaldson was last seen leaving an address in the Hilton Court area of Rosyth at around 2.10pm on Friday, March 26.
Lisa, who has links to the Kirkcaldy and Glenrothes areas, has not been seen since and police say “concerns are growing for her welfare.”
She is described as white, 4ft 11ins tall, of slim build with long black hair.
She was last seen wearing a cream parka style jacket, purple Adidas jumper, purple leggings and purple and pink trainers.
A police spokesperson said: “Extensive enquiries are underway to locate Lisa, however, attempts have so far proved unsuccessful.
“Anyone who has seen or has any knowledge or information regarding her whereabouts, is urged to contact police via 101, quoting incident number 1677 of Friday, 26 March, 2021.”