The social conversations of more than three million people were analysed, with Birmingham being crowned the winner.
25 towns and cities across the UK were analysed and combined with consumer research, a top five list was created - which no Scottish town managed to get on to.
Birmingham, home to comedians like Jo Enright and Frank Skinner, came out at number one.
Newcastle just scrapped in to the number five spot - the most northern location on the list.
Fabio Cannavale, of lastminute.com the team behind the study , said: “The British sense of humour is very unique so it’s very interesting to see what makes the UK giggle and even more so the contrast in humour between different towns and cities.”
Top 5 funniest cities