Harlequins' James Lang and Edinburgh's Darcy Graham join Scotland squad
Glasgow centre Johnson sustained a knee injury during his side’s weekend defeat to Munster, while Scott is suffering from concussion.
Lang, 23, made his first two Scotland appearances during the summer wins over Canada and Argentina.
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Hide AdThe Scotland medical team have also confirmed that back-row forwards Magnus Bradbury (shoulder), David Denton (head) and Luke Crosbie (jaw) have returned to their clubs for treatment.
Uncapped Edinburgh back Darcy Graham has been promoted to a full squad member for the autumn Tests after training with the squad.