Holt has spent the last few days in hospital and is progressing well, according to The Daily Record, but has yet to be released.
It means Holt's assistant, David Martindale will take charge for the home clash against Jim Goodwin's men.
The players had been made aware on Friday of their boss' situation, with Martindale taking training.
Livingston released a statement which said: "Manager Gary Holt will not be in the dugout today due to illness.?
"?We wish him a speedy recovery and expect him to be back taking training on Monday.?
"?The club will be making no further comment and ask to respect Gary and his family’s privacy.?"
Livingston currently sit in third in the table after three games.