The punter from Dundee has lost out on the seven figure prize after the 180 day deadline for claiming it ran out on Sunday.
Their ticket won a £1 million prize in the EuroMillions UK Millionaire Maker draw on November 14 last year. The winning code was TZFN 99040.
The money they missed out on will now go to lottery-funded community projects.
Lotto bosses made a number of public appeals to find the latest winner but no one came forward to claim the cash.
Andy Carter, Senior Winners’ Advisor at The National Lottery said: “Unfortunately, I can confirm that the ticket-holder did not come forward within the deadline to claim their prize and has now sadly missed out on this substantial amount of money.
“To avoid this unfortunate situation happening again, I would urge all National Lottery players to check their tickets on a regular basis.
“We tried very hard to find the ticket-holder and it’s a real shame that they have missed out, but there is still one winner – the nation.
“This money, and all the interest earned over the 180 days, will now go to benefit projects all over the UK funded by The National Lottery.”
EuroMillions UK Millionaire Maker guarantees to create two millionaires in every draw.
For every EuroMillions line played, UK players automatically receive a Millionaire Maker code printed on their ticket.
The biggest unclaimed jackpot in lottery history - £9,476,995 - went to good causes in 2006.