Colin, 71, and Christine Weir, 62, scooped the EuroMillions eight years ago, allowing them to become the 22nd richest people in Scotland.
It is understood the Ayrshire couple have been living apart for nearly a year.
In a statement to The Sun they said they were “applying for divorce amicably”.
It read: “It is with deep regret that Chris and Colin Weir confirm they have been living apart for some time and intend to divorce amicably.
“They ask that the family’s privacy is respected and this time and there will be no further comment.”
The Sun reported it had emerged the couple had been living in separate mansions.
Colin had apparently signed over a property the couple had bought together in Troon to his wife.
He is understood to have bought a £1.5m waterfront home while Christine had sole ownership of the £3m Frognal House.
A local is reported as saying: “It’s a real shame because they’ve obviously been through so much together.”
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