The 1939 Dandy Monster Comic, which was published at the end of the preceding year, was the first of 81 editions of the annual.
It had a pre-sale estimate of £1,200 to £1,500 at Keys Fine Art Auctioneers in Aylsham, Norfolk, and the winning online bid at Thursday’s auction was for £1,250.
The annual, which had an original cover price of 2/6, features many of the characters that went on to become household comic names such as Desperate Dan and Korky The Cat.
The Dandy comic was first published by Dundee-based DC Thomson in December 1937 and continued until December 2012 in printed form, then for a further six months online.
Keys head of books Andrew Lindsay-Bullock said: “It is very rare to see a copy of the first ever Dandy annual, especially in such good condition.”