The European and External Relations Committee will also look at David Cameron’s agenda for reforming the UK’s relationship with the EU and the involvement of the Scottish Parliament and Scottish ministers in the negotiations.
MSPs are seeking views from individuals and organisations on the referendum and the reform negotiations, as well as the effectiveness of inter-governmental relations.
Launching the inquiry, committee convener Christina McKelvie said: “The UK Government’s referendum and reform agenda are likely to have profound implications for Scotland’s own relationship with the EU.
“With Europe increasingly dominating the political agenda, it is important that the Scottish Parliament plays an active role in the debate and provides a forum for the people of Scotland to have their say.”
Deputy convener Hanzala Malik said: “The outcomes of the negotiations on reform of the EU are likely to be an influential factor in the result of the referendum.
“The committee will look closely at the negotiations as they develop, but in particular we are seeking views as to what the implications may be for Scotland.
“We also want to ensure that the people of Scotland have their voices heard in Brussels and London.
“We will be looking at what arrangements are in place for Scottish ministers and parliamentarians to be consulted and informed, both during these negotiations and on other talks where they relate directly to devolved policy matters.