The incident happened on the A92 near Rathillet Cupar and was reported to the police at around 3.15pm.
Local reports have suggested that the lorry was found lying on its side on Friday morning at around 6.30am.
Police have said that a 55-year-old male driver was taken to Ninewells Hospital ‘as a precaution’ following the incident.
A Police Scotland spokesperson said: "Around 3.15am on Friday, 4 February, officers were called to reports of a single vehicle crash involving an HGV on the A92, near Rathillet, Cupar.
"The 55-year-old male driver was taken to Ninewells Hospital as a precaution."
It is understood that traffic is coping relatively well but a number of drivers have stopped to help.
People are being asked to ‘watch the approach’ if they are passing the area.