The van initially wrote ‘#ResignSturegon’ before hastily changing it after being widely shared online.
It has carried many slogans including ‘Resign Sturgeon’ and ‘We Don't Believe You Nicola’.
It was organised by group calling themselves Majority Scots which is run by Mark and Mary Devlin.
Mark explained that they decided to run the signs after the Committee on the Scottish Government Handling of Harassment Complaints claimed that the First Minister broke the ministerial code.
On Monday, the Hamilton Inquiry came to a contrary conclusion, stating that Ms Sturgeon did not breach the code.
Today, Ms Sturgeon faced a vote of no confidence in Parliament, which she survived with 65 votes to 31, with 27 abstentions.
Holyrood is now to break for the election campaigns which will start tomorrow.
The Scottish Parliament elections will be held in May.