Stars who have lent their support to the Let’s Stay Together film include Doctor Who and Torchwood star John Barrowman, EastEnders actor Ross Kemp, comic Eddie Izzard and actor Tony Robinson.
Other familiar faces urging Scots to vote against independence include campaigner Doreen Lawrence, the mother of murdered teenager Stephen Lawrence, One Foot In The Grave actor Richard Wilson and soap star Michelle Collins.
Campaign organisers said the views of people in England, Wales and Northern Ireland have largely gone unheard in the debate and the video will “show Scotland that we do care”.
Spokesman Andrew McGuinness said: “On 18 September Scotland will vote on whether to stay in the United Kingdom, or leave to form a separate country.
“For everyone in the rest of the UK, just as for Scotland, the result of the referendum will have a fundamental impact on our identity, our culture and our future.
“It is for the people of Scotland to decide, but we want them to know the true feelings of the vast majority of their fellow citizens of the United Kingdom who want us to stay together.”
The film follows the launch of an online campaign video for Yes Scotland featuring the Emmy award-winning Brian Cox, fellow actor Martin Compston, comedienne Elaine C Smith, Billy Elliot star Gary Lewis, Deacon Blue front-man Ricky Ross and double Michelin star chef Andrew Fairlie.