The nine-page document was released ahead of evidence to House of Representatives investigators from Joseph Maguire, the acting director of national intelligence.
In the 25 July phone call, Mr Trump asked Ukraine president Volodymyr Zelensky for “a favour” to investigate corruption claims involving the son of Joe Biden, the leading Democratic presidential hopeful.
It will also complicate the president's effort to characterise the findings as those of a lone partisan out to undermine him. Mr Trump insisted afresh that it is all political.
After the complaint was released, he immediately tweeted in capital letters: "The Democrats are trying to destroy the Republican Party and all that it stands for. Stick together, play their game and fight hard Republicans. Our country is at stake."
The White House released what it called “a rough” transcript of that call on Wednesday.
House Democrats who are now mulling Mr Trump's impeachment are hoping Joseph Maguire will explain why he withheld it from Congress for weeks.