The Prime Minister has previously insisted that the issue of Scottish independence was “settled for a generation” in 2014, but told MPs it was a risk if voters choose Brexit on June 23.
At Prime Minister’s Questions, he warned: “If you love your country, you don’t want to act in a way that could lead to its breakup.”
Mr Cameron also raised the possibility of a second independence referendum during a live television Q&A last night, telling a studio audience he was “worried” about Brexit leading to the break-up of the UK.
However, the Prime Minister has the power to block any bid for a second referendum, and following PMQs a Downing Street spokesperson refused to rule out doing so.
“That’s a hypothetical question,” the source said when asked if Mr Cameron’s stance on allowing a second referendum would change in the event of Brexit.