Five Sisters Zoo in West Calder, West Lothian, hopes to ship the male lions over from Belgium, where they were confiscated by government officials in May.
The zoo aims to stump up £150,000 over the next six months to enable it to house the lions and already has them pegged for a two-acre enclosure on site.
The law in Belgium forbids the use of wild animals in circuses.
Brian and Shirley Curran, owners of the Five Sisters Zoo, said: “This is an enormous undertaking for us but with all the pledges of help we have had we are confident that we can make this happen.
“We had not intended asking for public donations but the overwhelming response was that the public want to help and for this we are so grateful.”