Evidence of nest disturbances and the unexplained disappearance of adult buzzards and goshawks in Moy Forest, Tomatin, was reported to police in May.
The illegal activity is likely to have taken place over a number of weeks, with four buzzard nests being abandoned last month.
A further nest site containing breeding goshawks has also been abandoned without reason.
Inspector Mike Middlehurst, wildlife crime officer, said: “Raptor persecution is an ongoing issue for the Highlands and we need the public to come forward and work with us to try and eradicate this blight on the Highlands.
“Police Scotland with partners in the Highland Partnership for Action Against Wildlife Crime has worked to increase public confidence in reporting wildlife offences which has resulted in increased reporting.”
The forest is managed by Forestry Enterprise Scotland (FES), which has been monitoring the vulnerable nest sites.
FES environment manager Giles Brockman said: “I am really disappointed to see this case of deliberate persecution on the national forest estate.
“Forest Enterprise Scotland will work closely with partners to stop this unacceptable practice.”
Detective Chief Superintendent Sean Scott, Police Scotland’s wildlife crime lead, said: “Police Scotland is committed to combating wildlife crime, working closely with partners nationally and locally to tackle the persecution of Scotland’s iconic birds of prey.
“We will continue to use the latest available investigative techniques and tools to pursue those who commit raptor crime.”
Members of the public can report any suspicious circumstances or activities to Police Scotland on 101 or anonymously through Crime Stoppers 0800 555 111.