Around 40,000 people have taken part in the All Under One Banner gathering which set off from Kelvingrove Park at 11:30 before heading to Glasgow Green.
Organisers of the All Under One Banner event in Glasgow had said before the event that they anticipated 40,000 people would take part, although some campaigners on social media suggested the total turnout could be as high as 90,000.
Men, women and children were draped in Saltires and waving flags as they walked from Kelvingrove to Glasgow Green, where they heard from speakers including controversial former MSP Tommy Sheridan.
SNP MSP Stuart McMillan said he had received some abuse from pro-Union campaigners.
The Greenock and Inverclyde MSP said on Twitter: “On the Indi march and just had a nazi salute and the middle finger from your supporters @scotlandinunion. Just letting you know.”
Amanda McGinley took part in in the march with her daughter Ellie Casson, with both women sporting Saltire bows in their hair.
Ms McGinley, 51, said: “We’re just basically fighting for the cause. We’re serial marchers.”
Lesley Crawford, 56, said she had come to “show support”.
She stated: “There is still a lot of people who are still campaigning for a vote for independence, and we’re not going away.”
Although Scots voted to stay part of the UK by 55% to 45% in the 2014 referendum, marcher Geoff Craig, 53 was confident independence campaigners could win a second referendum.
He said that level of support for Scotland leaving the UK was “a good place to start from” and added backing for independence was “edging up”.
Nicola Sturgeon appeared to show her support for the demonstration, tweeting a thumbs up emoji when sharing a report of the march.