Breakthrough in breast cancer diagnosis with simple blood test

Mammograms could be rendered almost obsolete by a simple blood test

Cancer cells produce proteins called antigens that trigger the body to make antibodies against them – autoantibodies.

Researchers at the University of Nottingham have found that these tumour-associated antigens (TAAs) are good indicators of cancer, and now they have developed panels of TAAs that are known already to be associated with breast cancer to detect whether or not there are autoantibodies against them in blood samples taken from patients.

In a pilot study the researchers, who are part of the Centre of Excellence for Autoimmunity in Cancer (CEAC) group at the School of Medicine, University of Nottingham, took blood samples from 90 breast cancer patients at the time they were diagnosed with breast cancer and matched them with samples taken from 90 patients without breast cancer.

They used screening technology (protein microarray) that allowed them to screen the blood samples rapidly for the presence of autoantibodies against 40 TAAs associated with breast cancer, and also 27 TAAs that were not known to be linked with the disease.

Presenting the research at the 2019 NCRI Conference in Glasgow, Daniyah Alfattani, a PhD student in the group, said: “The results of our study showed that breast cancer does induce autoantibodies against panels of specific tumour-associated antigens.

“We were able to detect cancer with reasonable accuracy by identifying these autoantibodies in the blood.”

The researchers identified three panels of TAAs against which to test for autoantibodies.

The accuracy of the test improved in the panels that contained more TAAs. The panel of five TAAs correctly detected breast cancer in 29 per cent of the samples from the cancer patients and correctly identified 84 per cent of the control samples as being cancer-free. The panel of seven TAAs correctly identified cancer in 35 per cent of cancer samples and no cancer in 79 per cent of control samples.

The panel of nine antigens correctly identified cancer in 37 per cent of cancer samples and no cancer in 79 per cent of the controls.

“We need to develop and further validate this test,” said Alfattani. “However, these results are encouraging and indicate that it’s possible to detect a signal for early breast cancer. Once we have improved the accuracy of the test, then it opens the possibility of using a simple blood test to improve early detection of the disease.”

The researchers are now testing samples from 800 patients against a panel of nine TAAs, and they expect the accuracy of the test to improve with these larger numbers.

Alfattani added: “A blood test for early breast cancer detection would be cost- effective, which would be of particular value in low and middle income countries. It would also be an easier screening method to implement compared to current methods, such as mammography.”

The researchers estimate that, with a fully-funded development programme, the test might become available in the clinic in about four to five years. A similar test for lung cancer is currently being tested in a randomised controlled trial in Scotland, involving 12,000 people at high risk of developing lung cancer because they smoke. Participants who test positive for autoantibodies are then followed up with a CT scan every two yearsto detect lung cancer in its early stages when it is easier to treat.

The CEAC group is also working on similar tests for pancreatic, colorectal and liver cancers. Solid tumours like these, as well as lung and breast cancer, represent around 70 per cent of all cancers.

“A blood test capable of detecting any of these cancers at an early stage is the over-riding objective of our work,” concluded Alfattani.