The Grant/Gordon family own Glenfiddich, and are now worth a staggering £2.6 billion - increased from £1.9bn just a year ago.
They appeared on the latest Sunday Times Rich List for Scotland.
There is now a record 10 billionaires in Scotland, with Sir Ian Wood ranked as the fourth wealthiest in Scotland and the 72nd in the UK, with a combined family fortune of £1.44bn.
Owners of DC Thomson media, the Thomson family, are fifth on the Scottish list with £1.28bn, sharing their place with Buckie born family Trond Mohn and his sister Marit Mohn Westlake.
The Duke of Sutherland is ranked 15th in Scotland with £580 million.
North east businessman Steven Ferguson, worth £250m, has made the list after selling the Kintore based Ferguson Group for £320m.
A new entry to the UKs top 1,000 richest is Oban born Susie Wolff and her husband Toto, who are 23rd equal in Scotland with £240m after an investment in Formula One racing. Banchory born Pete Cashmore who founded the website Mashable, is valued at £170m.
Many north east oil and gas figures also made the list. Tom Cross, CEO of oil exploration and production firm Parkmead Group, featured with a fortune worth £150m.
The global top 10
1. David and Simon Reuben £13.1bn (up £3.4bn); Property, internet
2. Sri and Gopi Hinduja £13bn; Industry and finance
3. Len Blavatnik £11.6bn (down £1.58bn); Music, media and investment
4. Galen and George Weston and family £11bn; Retailing
5. Ernesto and Kirsty Bertarelli £9.78bn (up £330m); Pharmaceuticals
6. The Duke of Westminster £9.35bn (up £790m); Property
7. Charlene de Carvalho-Heineken and Michel de Carvalho £9.15bn; (up £2bn) Inheritance
8. Kirsten and Jorn Rausing £8.7bn; Inheritance, investment
9. Hans Rausing and family £8.6bn (up £2.2bn); Packaging
10. Alisher Usmanov £7.58bn (down £2.22bn); Investment