The state-backed lender has axed about 2,700 posts since March, and it is understood that IT and back-office positions in Edinburgh, Birmingham, London and Manchester will be affected by the latest cutbacks.
RBS said: “We understand how difficult this is for our staff and will be offering as much support as we can including redeployment to other roles where possible.”
The moves come as the Edinburgh-based bank seeks to slim down from a sprawling international bank to a UK-focused lender.
RBS saw its pre-tax losses swell to £968 million in the first quarter – more than double last year’s figure of £459m. The wider loss reflected the impact of a £1.2 billion payment to the Treasury to buy out a crucial part of its £45bn taxpayer bailout.