Sir Anthony is a keen painter and the exhibition will consist mainly of portraits and landscapes.
The 50 paintings will be exhibited at The Dome bar in Edinburgh for five days in March.
Sir Anthony, best known for his role as serial killer Hannibal Lecter in Silence of the Lambs, said: "When I paint, I just paint freely without anxiety regarding outside opinions as criticisms. I do it for sheer pleasure.
"It's done wonders for my for my sub conscious. I dream now in colours".
The organiser of the exhibition, Jonathan Poole, said: "It is very exciting to have Sir Anthony Hopkins' work. This exhibition gives people the chance to see a different side of Sir Anthony in terms of his artistry and also his unique personality."
In 2006, Sir Anthony's paintings shown at a California gallery sold for more than $6,000 (3,700).
The Welsh actor's next film – The Wolfman, also starring Emily Blunt and Benicio del Toro – is due for release in cinemas next month.
After opening in London on 16 February, the collection will move to Edinburgh on 2 March.