Boero, 38, was nearing the end of a special section among dunes on the Atlantic coast on his Beta RR450 on the stage from Mar del Plata in Buenos Aires province to Santa Rosa in La Pampa in the centre of the country, organisers said.
The son of a former national motor racing champion and taking part in his second Dakar rally, he suffered serious injuries to his thorax and cardiac arrest.
“The rider suffered a cardiac arrest following his fall and was attended five minutes after the accident by the medical staff, who reached the place of the incident by helicopter,” said a statement on the race website.
“Despite their best efforts, the doctors were unable to resuscitate the pilot, who died while he was being taken to hospital.”
The rally, which moved from Africa to South America for security reasons four years ago, is notoriously dangerous and has claimed several lives in the past.