As revealed in Tuesday's Evening News, the Hearts winger hoped to feature at Easter Road having missed two matches with a torn thigh muscle. He underwent intensive physiotherapy last weekend in an attempt to return to training and force himself into contention.
However, he broke down after rejoining full training and has now been sidelined for the rest of the campaign. His absence strikes another blow to Hearts' European aspirations for next season but the 22-year-old will attempt to rest between now and the end of June before beginning pre-season training.
Marius Zaliukas will continue as skipper against Hibernian in place of Hearts' club captain Michael Stewart. Manager Jim Jefferies is keen to play down the fact that Stewart, out of contract in June, is no longer in possession of the armband.
"I have said before there is nothing sinister in this. Marius had been doing the job fine and I wanted Michael to concentrate on his football as he had been out for a few weeks injured," said Jefferies.
"Marius will continue as captain this weekend if he is selected but to be honest I want 11 captains out on that park. It's really not something that concerns me too much at the moment and it's not a major issue.
"We have a couple of matches left after the Hibs game and it could be that I might even look at a couple of others in those games and see how they handle it and what they offer."
Lee Wallace and Suso were expected to resume training today after sustaining minor knocks during last weekend's match against Motherwell.