Lorna Slater, the newly elected co-leader of the Greens, says it wouldn't "make sense" to agree deal on the wording in each party's manifesto to push for a second referendum in the 2021 Holyrood elections.
It comes after a new poll today shows a majority of Scots now back leaving the UK.
But Ms Slater told the Herald: “I don’t think it does make sense. It depends what you mean by co-operate with.
“We’re two very different political parties that have only one policy in common.
“So I don’t think it makes sense to sacrifice everything we believe in for that one policy.”
She added: “I expect our manifesto will call boldly for an independence referendum.”
Ms Slater was elected co-leader of the Scottish Greens alongside long-serving MSP Patrick Harvie last week.