Ministers said £1.14 million has been allocated for 2019/2020 from the Scottish Government’s £80 million active travel budget to finance the e-bike grant fund.
The scheme, delivered by the Energy Saving Trust, aims to promote e-bikes as more sustainable alternatives to car journeys.
Public and third sector organisations are invited to apply for cash to support the creation of bike pools, public hire and loan schemes.
Applications are encouraged from councils, public sector agencies, further and higher education institutions, active travel hubs and community groups.
Up to £200,000 per application is now available to help organisations and communities access e-bikes in a more affordable way.
Transport Secretary Michael Matheson said: “The advances in technology mean that e-bikes can provide a more sustainable alternative to single-occupancy car journeys. The only downside is that they still remain expensive for many and high costs remains a barrier to uptake.
“This is where I’m proud the Scottish Government has helped to provide affordable access through this progressive initiative. Our previous rounds will introduce 460 e-bikes across Scotland, including access through high-impact cycle hire schemes.
“Through this new round I hope that even more organisations right across Scotland choose to apply, so that we increase opportunities for people to discover this exciting way to travel and secure profound benefits for our air quality, health and sense of wellbeing.”