The First Minister called on the thousands of spectators to show their solidarity with those affected by the tragedy.
Mr Salmond said: “In a few minutes’ time we will hear the words of Prince Imran as he introduces Her Majesty the Queen as head of the Commonwealth.
“He is not only the president of the games federation, he is a Prince of Malaysia - a nation which has been touched by great tragedy in recent days.”
He added: “In last week’s disaster almost one third of the 298 victims were citizens of the Commonwealth, 193 came from our European neighbours in The Netherlands.
“Please rise now, please stand, as we observe a moment’s silence to express our condolences and above all our solidarity with the loved ones of all of those of every nationality who lost their lives.”
The First Minister went on to welcome the Commonwealth nations to Scotland, before the audience heard from Glasgow City Council leader Gordon Matheson.
He said: “While you’ve been training and qualifying, or saving and making travel plans, the people of Glasgow have been getting ready to welcome you.
“Everything is in place so that you can perform at your best and have the time of your life in this beautiful and friendly city.
“The people of Glasgow are deeply grateful to the Commonwealth family, the Glasgow 2014 Organising Committee, and all our partners and friends who have worked with us.
“And we are especially delighted and honoured that Her Majesty the Queen, and His Royal Highness, The Duke of Edinburgh, are present.
“And so, we are gathered here in this this stadium and in front of television screens across the city and throughout the world. Our time has come. Let Glasgow Flourish. And as for our Games. Bring it on.”