Police said an entire trailer was taken from Nether Southbar Farm on Greenock Road in Inchinnan – a small village in Renfrewshire – around 1am on Sunday.
The Southbar Transport trailer contained bottles of whisky and gin worth a six figure sum.
It follows a similar theft from a haulage firm in nearby Linwood last week when a similar value of alcohol was taken.
Two men wearing hi-vis clothing entered a yard in Burnbrae Road on 9 December and gained access to two trucks.
Police said the incidents were not being linked at this time, but enquiries were continuing.
On the most recent theft, Detective Inspector Ross MacDonald said: “Enquiries are at an early stage into this high-value theft.
“Initial enquiries have revealed that two white trucks were seen in the area around the time of the theft and we are currently carrying out enquiries to establish if there were involved in this incident.
“At this time I would urge anyone who was in the area early on Sunday morning who either witnessed anything suspicious, or who has any information that may assist our investigation to contact Paisley Police Office through 101.”