Police, including specialist resources, were called to the northbound M90 near Dunfermline, Fife, at around 7.45pm on Wednesday.
Eye witnesses told of long delays on the motorway and a helictoper flying overhead as officers dealt with the incident.
A male teenager, aged 17, and a 27-year-old man have been arrested in connection with the incident.
A report will be sent to the procurator fiscal.
Officers said they are keen to hear from witnesses and anyone who may have video footage that could help their inquiry.
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