The three-year deal involves the firm taking more than 4,000sq ft at a rate of 14.50 per square foot.
Established in 2007, H2Ology holds a number of patents in conjunction with Edinburgh University covering the Advanced Treatment Station (ATS) family of products.
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Hide AdThe company's divisions encompass a range of water-related services from waste water discharge, recycling and management to usage minimisation.
Richard Palmer, chief executive at H2Ology said: "The H2Ology team and I are delighted to be moving our core activities to Edinburgh Technopole.
"As well as becoming H2O's head office, the facilities will become our UK-wide research and development centre, which is particularly appropriate bearing in mind our existing worldwide patent programme in conjunction with Edinburgh University."
Edinburgh Technopole is a joint venture between Edinburgh University and property developer Grosvenor.
The 500,000sq ft site on the Bush Estate, to the south of Edinburgh, includes a mixture of office and laboratory space designed to attract science companies.